Department of Hemato-Oncology, University Hospital Pilsen and Biomedical Center, Faculty of Medicine in Pilsen presents on May 3, 2018 from 13:00 at Seminar room (SO 01. 2.21), Biomedical Center, alej Svobody 1655/76, Plzeň 323 00 lectures from leading scientist from Imperial College London.

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Biomedical Center, Faculty of Medicine in Pilsen, Charles University, is seeking students for the following Ph.D. study topics in the doctoral study programme of Experimental Surgery:

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Team of Laboratory of Cancer Treatment and Tissue Regeneration participates on research of GECCO (The Genetics and Epidemiology of Colorecal Cancer Consortium) in Seattle, USA.

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Recently, we published a review article entitled “A Double Edged Sword role of Interleukin-22 in Wound Healing and Tissue Regeneration” in prestigious journal ‘Frontiers in Immunology’. Frontiers in Immunology is the official Journal of the International Union of Immunological Societies (IUIS). It is a leading journal in its field, publishing rigorously peer-reviewed research across basic, translational and clinical immunology.

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26th annual prizes of the French embassy of the Czech Republic for young czech scientists in postgraduate studying programs were announced this year. These were given in seven categories according to the scientific discipline of the nominees. Two students of our faculty were awarded the Albert Schweitzer prize for medical research. Student Jachym Rosendorf, M.D. was awarded a 3rd place prize for his research „Experimental use of nanofibrous materials for prevention of intestinal anastomotic leakage“ and Richard Palek, M.D. was awarded a 2nd place prize for his work on „Perfusion decellularization of whole liver - first step in new organ development“. Both of them are students of Assoc. Prof. Vaclav Liska, M.D., PhD, who is an Albert Schweitzer prize awardee himself for the year 2000. The students worked on their projects under his supervision at the Laboratory of Cancer Treatment and Tissue Regeneration of the Biomedical Center. The awarding Ceremony was held on 2nd July in the facility of the French Institute in Prague in presence of the French ambassador Roland Galharague. The opening speech is annually given by a chemistry nobelist Jean-Marie Lehn, who was able to participate only electronically this year due to the epidemiological situation. The financial award is accompanied by a month internship in a research facility in France for the first and the second prize awardees sponsored by the Pierre-Fabre Medicament company. We congratulate the awardees.

MUDr. Jan Brůha, Ph.D.

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Pavel Vodicka, M.D., PhD and Alena Opattova, PhD, from Laboratory of Cancer Treatment and Tissue Regeneration Bimedical Center in Pilsen and Institute of Experimental Medicine of the Czech Academy of Sciences, Prague are coauthors of two scientific articles published in in Nature Protocols (IF 11.360).

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Oncological and surgical treatment of malignant focal processes of the liver reaches its limits in cases where the amount of affected parenchyma is too large or where the hilar structures are involved – stages at which a complete removal of the tumor tissue equals a total loss of the organ. In such patients, restoration of the liver function by means of conventional liver transplantation is generally out of the question, as it requires the administration of drugs to suppress the immune system’s response. Since the immune system fights not only pathogens but also cancerous cells, immunosuppression can lead to a rapid recurrence of the disease originating from the remnants of the malignant cells left in the body. The hope for a change lies in tissue engineering research, which promises to create an artificial replacement organ using the patient's own cells, thereby reducing the need for immunosuppression [Liska2019]. Our Laboratory of Cancer Treatment and Tissue Regeneration, headed by Professor Václav Liška, located at the Biomedical Center of the Faculty of Medicine in Pilsen, is engaged in such research.


 Liver scaffold in the final stage of preparation

An important step in our approach to the production of an artificial organ is the preparation of an inanimate skeleton of the liver (scaffold), which is obtained from the liver of a domestic pig and is intended for subsequent colonization by living cells. To evaluate the quality of this scaffold, it is necessary to distinguish the areas of the liver lobules and their surroundings (septa). To follow the quality assessment methodology described in [Moulisova2020], it has hitherto been necessary to manually label parts of the scaffold samples in histological slides stained with hematoxylin and eosin. In cooperation with colleagues from the Department of Cybernetics of the Faculty of Applied Sciences at the University of West Bohemia in Pilsen and Universitätsklinikum Jena, we designed an algorithm that produces the label automatically. The algorithm, which is based on convolutional neural networks, was published ( in the Sensors journal (Q1, IF=3.275).

003 optimized

Hematoxylin-and-eosin-stained liver scaffold

Compared to classical methods of computer vision, convolutional neural networks usually offer better results, but require a considerable amount of training data. Therefore, we designed a two-phase training procedure. In the first phase, we use the methods of classical computer vision for semi-automatic segmentation using texture features. In this phase, we use training datasets where less than 5% of the image area is annotated. The output data are then used in the second phase for training a neural network. The architecture of the neural network is inspired by the U-Net network. After the training, full segmentation is produced automatically.



Two-phase training procedure

Automatic histological image processing allows extensive skeletal quality measurements to be made. An important benefit of this method is the repeatability of measurements and their independence from the human factor, once the network is trained. In the follow-up research, our team is going to integrate the proposed method into a freely available application, which will make it possible to compare the results of laboratories from all over the world.


M. Jiřík, I. Gruber, V. Moulisová, V. Liška et al.






 Liška V, Moulisová V, Pálek R, Rosendorf J, Červenková L, Bolek L, Třeška V. Repopulation of decellularized pig scaffolds: Promising approach for liver tissue engineering. Rozhl Chir. 2019 Fall;98(10):388-393. English. PMID: 31842567.


Moulisová V, Jiřík M, Schindler C, et al. Novel morphological multi-scale evaluation system for quality assessment of decellularized liver scaffolds. Journal of Tissue Engineering. January 2020. doi:10.1177/2041731420921121.


 Jirik, M.; Gruber, I.; Moulisova, V.; Schindler, C.; Cervenkova, L.; Palek, R.; Rosendorf, J.; Arlt, J.; Bolek, L.; Dejmek, J.; Dahmen, U.; Zelezny, M.; Liska, V. Semantic Segmentation of Intralobular and Extralobular Tissue from Liver Scaffold H&E Images. Sensors 2020, 20, 7063.

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Teams from the Laboratory of Cancer treatment and tissue regeneration of the Biomedical Center in Pilsen and from the Department of nonwovens and nanofibrous materials of the Technical University in Liberec cooperate on development of nanofibrous materials for healing support of intestinal anastomoses for several years.

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The Laboratory of Reproductive Medicine presents prof. Jean-Francois BODART, visiting professor at Faculty of Medicine in Pilsen, and his lecture entitled O-GlcNAcylation & S-Sulfhydration: sweet and smelly sides of cell cycle regulation.

How to modulate cell cycle and its progression through post-translational modifications of crutial proteins? Recently, the creation and role of certain post-translational modifications remain still unclear while the utilization of this knowledge is essential for drug investigation and cure. The future of medicine belongs to post-translational modifications!

Thursday 14th September 2017, 3 p.m., seminar room of Biomedical center

The lecture will be about 30 min., accompanied by small refreshment

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The Laboratory of Reproductive Medicine
presents prof. Peter Šutovský, Ph.D., Dr. h. c.
visiting professor at Faculty of Medicine in Pilsen
and his lecture entitled
Post-fertilization Sperm Mitophagy: The Tale of Mitochondrial Eve and Steve.

Tuesday 17th October, 2:40 p.m., lecture hall, Prochaskuv pavilon (PRO)

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Faculty of Medicine in Pilsen, Charles University, invites you to a comprehensive 2-Day Stereology Workshop (April 24–25, 2018) led by Professor Peter R. Mouton, Ph.D. Chief Scientific Officer, Stereology Resource Center, Tampa, FL, USA & Director, Stereology Laboratory at the University of South Florida, Tampa, FL, USA and visiting professor of Charles University Applications of Unbiased Stereology to Biological Tissue.

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Dean of the Faculty of Medicine in Pilsen, Charles University announces the selection procedure for filling a job Post-Doc Researcher – Laboratory of Reproductive Medicine of the Biomedical Center – Beginning – September 2018 (full time job).

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The Laboratory of Reproductive Medicine presents a lecture by Dr. Petr Šolc – Institute of Animal Physiology and Genetics, Czech Academy of Sciences on Wednesday, May 9th, 2018, 10 a.m. in seminar room of Biomedical Center.

The Petr Solc’s lecture has been canceled. The lecture will be postponed and alternative term will be announced.

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From May 25 to May 27 2018 a meeting called «The Contribution of Pharmacogenomics to the Diagnosis and Treatment of Generalized Colorectal Carcinoma IX» took place at a lovely setting of a mountain hut at Javorná.

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Faculty of Medicine in Pilsen, Charles University, invites you to the seminar of the visiting professor of Charles University Dr. Václav Větvička, Ph.D. University of Louisville, Department of Pathology Louisville, Kentucky, USA.

Date: September 24th, 2018, 10:00–11:30. Venue: Biomedical center, Alej Svobody 76, Plzeň; seminar room, 1st floor

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Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
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