Laboratory of Reproductive Medicine

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About us

Laboratory of Reproductive medicine is focused on spermatogenesis, oocyte maturation, mechanisms of fertilization and pre-implanted embryonic development in vitro. Mice (Mus musculus) are utilized as a biological model allowing transfer of our results onto further research of human reproduction. In addition to in vitro experiments, mouse model offers in vivo testing of selected environmental polutants. Moreover, analysis of human gametes and body fluids of reproductive system takes place in Laboratory of Reproductive Medicine.

What are we focused on?

  • Epigenetic regulation of fertilization and early embryonic development.
  • Influences negatively affecting sperm quality and fertilization ability.
  • Role of ubiquitin-proteasomal system in epigenetics.
  • Involvement of hydrogen sulfide, one of gasotransmitters, in epigenetic changes.
  • Evaluation of impact of endocrine disruptors on mammalian reproduction.

What we can do?

  • In vitro oocyte maturation (IVM), in vitro fertilization (IVF) and embryo culture (IVC).
  • Flow cytometry and sorting of sperm (FACSAria, FACSVerse, BD Bioscience).
  • Intracytoplasmic sperm injection (ICSI).
  • Immunocytochemistry of oocytes and embryos.
  • Epi-fluorescent and confocal laser-scanning microscopy, live cell imaging (Olympus, IX83, Tokai Hit).
  • Western Blotting (Biorad).

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Biomedical Center
alej Svobody 1655/76
323 00 Pilsen – Severní Předměstí
Telefon: +420 377 593 808
E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it. 
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Contact

Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
E: This email address is being protected from spambots. You need JavaScript enabled to view it.

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