About us
Scientific activities of Laboratory of Antibiotic Resistance and Application of Mass spectrometry in Microbiology (ARAMM) are mainly focused on mechanisms of resistance to antibiotics, especially to expanded-spectrum cephalosporins and carbapenems, in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas spp.). The research projects aim to execute molecular epidemiology of factors affecting dissemination of multi-drug resistance among nosocomial pathogens. Methodological approaches, also, include analysis of genetic carriers of resistance determinants and whole-genome sequencing. The second main objective of the research projects is intending in the development of new techniques for the detection and study of resistance mechanisms using MALDI-TOF mass spectrometry and Raman spectroscopy. In 2011, the members of our group demonstrated that MALDI-TOF mass spectrometry can directly detect carbapenemase activity. Also, recently, we patented a method for the identification of β-lactamases in clinical isolates by MALDI-TOF mass spectrometry, based on their molecular weight.
Releated Research themes
Contact
Biomedical Center
alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí
Members
- Prof. Jaroslav Hrabák, Ph.D., M.Sc. – Research Group Leader
- Jana Amlerová, M.D.
- Tamara Bergerová, M.D.
- Ibrahim Bitar, Ph.D.
- Monika Dolejská, Ph.D., M.Sc.
- Miroslava Chalupová, M.D.
- Kateřina Chudějová, M.Sc.
- Vladislav Jakubů, M.Sc.
- Helena Janouškovcová, M.D.
- Dana Králová, DiS.
- Lucie Kraftová, B.Sc.
- Constantinos Papagiannitsis, Ph.D.
- Veronika Pašková, M.Sc.
- Anna Skálová, M.D.
- Radka Walková, M.D.
- Assoc. Prof. Helena Žemličková, M.D., Ph.D.
We offer
- DNA sequencing, including capillary and whole-genome sequencing, and metagenomic analysis
- Molecular typing of Gram-negative bacteria
- Detection of resistance mechanisms in Gram-negative bacteria (β-lactamases, mechanisms of resistance in fluroquinolones, aminoglycosides, colistin, etc.)
- Applications of mass spectrometry in microbiology
- Applications of Raman spectroscopy in microbiology