Laboratory of Tumor Biology and Immunotherapy

News

About us

The cancer is one of the most common causes of death in the world. Over the last decades, a deep research in oncological field brought new drugs positively influence outcome of patients such as specific molecular inhibitors and immunotherapy. However, in spite of this great progress, many patients still die as a direct result of oncological disease, and thus new biomarkers and therapies are still being sought to improve patient outcomes.

The Laboratory of Tumor Biology and Immunotherapy studies tumor diseases (particularly haematological malignancies and colorectal carcinoma) to identify potential biomarkers to improve disease detection, prediction and prognosis and to identify processes and interactions that might help to develop or improve immunotherapy of these diseases.

 

We are focused on:

  • Development and testing of immunotherapies mainly for haematological malignancies
  • Tumor microenvironment with focus on immune cells and mesenchymal stem cells
  • Single cell analysis of tumor and immune cells in haematological malignancies
  • Liquid biopsies with focus on cell-free RNA in plasma

 

Our methods:

  • Processing of biological samples (MNCs cryopreservation, RNA/DNA isolation, serum/plasma collection)
  • Expression analysis (qPCR and mass spectrometry)
  • Sequencing – Sanger, NGS
  • Immunophenotyping – flow cytometry, immunochemistry
  • Cell culture of cell lines and primary cells

 

Used equipment:

  • NGS (MiSeq)
  • Sanger sequencing (3500 Genetic Analyzer)
  • qPCR (LightCycler 96 System, CFX96 Touch Real-Time PCR Detection System)
  • Flow cytometry (BD FACSAria Fusion)
  • Microscopy (Olympus IX83 with Yokogawa CSU W1 confocal head)
  • Live cell imaging (Incucyte Live-Cell Analysis System)
  • Microarrays (SureScan Microarray Scanner)
  • Mass spectrometer

 

International collaboration:

  • Prof. Peter Kuhn - USC Michelson Center for Convergent Bioscience, Los Angeles, CA
  • Dr. Valentina Caputo - Faculty of Medicine, Department of Immunology and Inflammation, Imperial College London, UK
  • Dr. Iros Barozzi - Faculty of Medicine, Department of Surgery & Cancer, Imperial College London, UK
  • Dr. Antonia Rotolo - University of Pennsylvania, University of Pennsylvania, Philadelphia, USA

 

National collaboration:

  • Department of Hematology-oncology, Faculty Hospital Pilsen
  • New Technologies for the Information Society, University of West Bohemia, Pilsen
  • The Czech National Marrow Donor Registry, Pilsen
  • Department of Internal Medicine, Faculty Hospital Pilsen
  • SOTIO company, Prague

Releated Research themes

Contact

Charles University
Faculty of Medicine in Pilsen
Biomedical Center
alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

Members

  • Dr. Monika Holubová - research group leader (immunotherapy, flow cytometry)
    This email address is being protected from spambots. You need JavaScript enabled to view it., 377 104 645
  • Dr. Pavel Ostašov - senior researcher (sequencing, liquid biopsies)
    This email address is being protected from spambots. You need JavaScript enabled to view it., 377 593 845
  • Dr. Pavel Pitule - post-doc researcher (qPCR, alternative splicing)
    This email address is being protected from spambots. You need JavaScript enabled to view it., 377 593 845
  • Dr. Iva Machová - post-doc researcher (mass spectrometry, protein analysis)
    This email address is being protected from spambots. You need JavaScript enabled to view it., 377 593 320
  • Dr. Miroslava Čedíková - post-doc researcher (pre-graduate students supervisor, immunotherapy)
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  • Eva Kralovcová - lab assistant (lab management, molecular biology)
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  • Pavlína Mrázová, BSc - lab assistant (cell culture, flow cytometry)
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PhD students:

  • Nazila Navvabi, MSc
  • Drahomír Kolenčík, MD
  • Alena Machuldová, MSc
  • Diana Macečková, MSc
  • Tereza Dekojová, MD
  • Robin Klieber, MSc

 

External workers:

  • Associate professor Martin Pešta
  • Jiří Polívka, MSc

Publications

  • Vycital O, Pitule P, Hosek P, Kriz T, Treska V, Liska V. Expression of Serpin B9 as a Prognostic Factor of Colorectal Cancer. Anticancer Res. 2019 Nov;39(11):6063-6066. doi: 10.21873/anticanres.13813
  • Fiala O, Ostasov P, Hosek P, Sorejs O, Liska V, Buchler T, Poprach A, Kucera R, Topolcan O, Sustr J, Sedivcova M, Finek J. The Predictive Role of Primary Tumour Sidedness in Metastatic Colorectal Cancer Treated With Targeted Agents. Anticancer Res. 2019 Oct;39(10):5645-5652. doi: 10.21873/anticanres.13761
  • Truxova I, Kasikova L, Salek C Hensler M, Lysak D, Holicek P, Bilkova P, Holubova M,……. Spisek R, Fucikova J. Calreticulin exposure on malignant blasts correlates with improved natural killer cell-mediated cytotoxicity in acute myeloid leukemia patients. Haematologica. 2019 Oct 3. pii: haematol.2019.223933. doi: 10.3324/haematol.2019.223933
  • Benjamin M. Tang, Maryam Shojaei, …….., Marek Nalos, Monika Holubova, Tracy Chew, David Booth, Anand Kumar, Anthony McLean & Klaus Schughart. NEUTROPHILS-RELATED HOST FACTORS ASSOCIATED WITH SEVERE DISEASE AND FATALITY IN PATIENTS WITH INFLUENZA INFECTION. Nature Communications,10, Article number: 3422 (2019) , DOI: 10.1038/s41467-019-11249-y
  • Holubova M, Leba M, Gmucova H, Caputo V, Jindra P and Lysak D. Improving the Clinical APPLICATION OF NATURAL KILLER CELLS BY MODULATING SIGNALS SIGNAL FROM TARGET CELLS. Int. J. Mol. Sci. 2019, 20, 3472; doi:10.3390/ijms20143472
  • P.Holicek, I.Truxova, L.Kasikova, S.Vosahlikova, C.Salek, J.Rakova, M.Holubova, D.Lysak, I.Cremer, R.Spiseka, J.Fucikova. ASSESSMENT OF NK CELL-MEDIATED CYTOTOXICITY BY FLOW CYTOMETRY AFTER RAPID, HIGH-YIELD ISOLATION FROM PERIPHERAL BLOOD. Methods in Enzymology. June 2019, doi.org/10.1016/bs.mie.2019.05.034
  • Thiele JA, Pitule P, Hicks J, Kuhn P. SINGLE-CELL ANALYSIS OF CIRCULATING TUMOR CELLS. Methods Mol Biol. 2019;1908:243-264. doi: 10.1007/978-1-4939-9004-7_17
  • Altanerova U, Jakubechova J, Benejova K, Priscakova P, Pesta M, Pitule P, Topolcan O, Kausitz J, Zduriencikova M, Repiska V, Altaner C. PRODRUG SUICIDE GENE THERAPY FOR CANCER TARGETED INTRACELLULAR BY MESENCHYMAL STEM CELL EXOSOMES. Int J Cancer. 2019 Feb 15;144(4):897-908. doi: 10.1002/ijc.31792
  • Miklíková M, Jarkovská D, Čedíková M, Švíglerová J, Kuncová J, Nalos L, Kubíková T, Liška V, Holubová M, Lysák D, Králíčková M, Vištejnová L, Štengl M. BENEFICIAL EFFECTS OF MESENCHYMAL STEM CELLS ON ADULT PORCINE CARDIOMYOCYTES IN NON-CONTACT CO-CULTURE. Physiol Res. 2018 Dec 31;67(Supplementum 4):S619-S631
  • Dejmek J, Kohoutová M, Kripnerová M, Čedíková M, Tůma Z, Babuška V, Bolek L, Kuncová J. REPEATED EXPOSURE TO HYPERBARIC HYPEROXIA AFFECTS MITOCHONDRIAL FUNCTIONS OF THE LUNG FIBROBLASTS. Physiol Res. 2018 Dec 31;67(Supplementum 4):S633-S643
  • Hlavac V, Kovacova M, Elsnerova K, Brynychova V, Kozevnikovova R, Raus K, Kopeckova K, Mestakova S, Vrana D, Gatek J, Ostasov P, Vaclavikova R, Soucek P. Use of Germline Genetic VARIABILITY FOR PREDICTION OF CHEMORESISTANCE AND PROGNOSIS OF BREAST CANCER PATIENTS. Cancers (Basel). 2018 Dec 12;10(12). pii:E511. doi: 10.3390/cancers10120511. PubMed PMID: 30545124
  • Rotolo A, Caputo VS, Holubova M, Baxan N, Dubois O, Chaudhry MS, Xiao X, Goudevenou K, Pitcher DS, Petevi K, Kachramanoglou C, Iles S, Naresh K, Maher J, Karadimitris A. ENHANCED ANTI-LYMPHOMA ACTIVITY OF CAR19-INKT CELLS UNDERPINNED BY DUAL CD19 AND CD1D TARGETING. Cancer Cell. 2018 Oct 8;34(4):596-610. PMID: 30300581
  • Thiele JA, Hosek P, Kralovcova E, Ostasov P, Liska V, Bruha J, Vycital O, Rosendorf J, Opattova A, Horak J, Kralickova M, Vodicka P, Pitule P. LNCRNAS IN NON-MALIGNANT TISSUE HAVE PROGNOSTIC VALUE IN COLORECTAL CANCER. Int J Mol Sci. 2018 Sep 8;19(9). pii: E2672. PubMed PMID: 30205577
  • Polívka J Jr, Pešta M, Pitule P, Hes O, Holubec L, Polívka J, Kubíková T, Tonar Z. IDH1 MUTATION IS ASSOCIATED WITH LOWER EXPRESSION OF VEGF BUT NOT MICROVESSEL FORMATION IN GLIOBLASTOMA MULTIFORME. Oncotarget. 2018 Feb 20;9(23):16462-16476. PMID: 29662659
  • Fikar P, Georgiev V, Lissorgues G, Holubova M, Lysak D, Georgiev D. 2DEP CYTOMETRY: DISTRIBUTED DIELECTROPHORETIC CYTOMETRY FOR LIVE CELL DIELECTRIC SIGNATURE MEASUREMENT ON POPULATION LEVEL. BIOMED MICRODEVICES. 2018 Jan 8;20(1):12

Contact

Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
E: This email address is being protected from spambots. You need JavaScript enabled to view it.

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