Laboratory of Tumor Biology and Immunotherapy

About us

The cancer is one of the most common causes of death in the world. Over the last decades, a deep research in oncological field brought new drugs positively influence outcome of patients such as specific molecular inhibitors and immunotherapy. However, in spite of this great progress, many patients still die as a direct result of oncological disease, and thus new biomarkers and therapies are still being sought to improve patient outcomes.

The Laboratory of Tumor Biology and Immunotherapy studies tumor diseases (particularly haematological malignancies and colorectal carcinoma) to identify potential biomarkers to improve disease detection, prediction and prognosis and to identify processes and interactions that might help to develop or improve immunotherapy of these diseases.

We are focused on:

  • Development and testing of immunotherapies mainly for haematological malignancies
  • Tumor microenvironment with focus on immune cells and mesenchymal stem cells
  • Single cell analysis of tumor and immune cells in haematological malignancies
  • Liquid biopsies with focus on cell-free RNA in plasma

Our methods:

  • Processing of biological samples (MNCs cryopreservation, RNA/DNA isolation, serum/plasma collection)
  • Expression analysis (qPCR and mass spectrometry)
  • Sequencing – Sanger, NGS
  • Immunophenotyping – flow cytometry, immunochemistry
  • Cell culture of cell lines and primary cells

Used equipment:

  • NGS (MiSeq)
  • Sanger sequencing (ABI 3500)
  • qPCR (Light Cycler 480, BioRad)
  • Flow cytometry (FACS ARIA Fusion)
  • Microscopy (Olympus IX83 with Yokogawa CSU W1 confocal head)
  • Live cell imaging (IncuCyte)
  • Microarray arrarys
  • Mass spectrometer

International collaboration:

  • Prof. Peter Kuhn - USC Michelson Center for Convergent Bioscience
  • Dr. Valentina Caputo - Faculty of Medicine, Department of Immunology and Inflammation, Imperial College London
  • Dr. Iros Barozzi - Faculty of Medicine, Department of Surgery & Cancer, Imperial College London
  • Dr. Antonia Rotolo - University of Pennsylvania

National collaboration:

  • Department of Hematology-oncology, Faculty Hospital Pilsen
  • New Technologies for the Information Society, University of West Bohemia
  • The Czech National Marrow Donor Registry
  • Department of Internal Medicine, Faculty Hospital Pilsen
  • SOTIO company, Prague

Releated Research themes

Contact

Biomedical Center
alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

Members

  • Dr. Monika Holubová - research group leader (immunotherapy, flow cytometry)
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  • Dr. Pavel Ostašov - senior researcher (sequencing, liquid biopsies)
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  • Dr. Pavel Pitule - post-doc researcher (qPCR, alternative splicing)
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  • Dr. Iva Machová - post-doc researcher (mass spectrometry)
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  • Dr. Miroslava Čedíková - post-doc researcher (pre-graduate students supervisor, immunotherapy)
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  • Eva Kralovcová - lab assistant (lab management, molecular biology)
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  • Pavlína Mrázová, BSc - lab assistant (cell culture, flow cytometry)
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  • PhD students:

    • Nazila Navvabi, MSc
    • Drahomír Kolenčík, MD
    • Alena Machuldová, MSc
    • Diana Macečková, MSc
    • Tereza Dekojová, MD
    • Robin Klieber, MSc

    External workers:

    • Associate professor Martin Pešta
    • Jiří Polívka, MSc

Publications and Patents

News

Contact

Charles University
Faculty of Medicine in Pilsen
Biomedical Center

alej Svobody 1655/76
323 00 Plzeň – Severní Předměstí

T: +420 377 593 810
E: This email address is being protected from spambots. You need JavaScript enabled to view it.

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