N. Abello, B. Barroso, H. A. M. Kerstjens, D. S. Postma, R. Bischoff, Chemical labeling and enrichment of nitrotyrosine-containing peptides
Talanta 2010, 80, 1503-1512.
given by Jiří Moravec
Protein tyrosine nitration (PTN) is a post-translational modification of proteins associated with a number of inflammatory diseases. Since PTN is a low-abundance post-translational modification, it is necessary to enrich modified proteins and/or to detect them with high selectivity and sensitivity. Until now, this has been mostly accomplished with anti-nitrotyrosine antibodies in combination with two-dimensional gel electrophoresis and mass spectrometry.
We propose a chemical labeling approach designed to allow enrichment of tyrosine-nitrated peptides independent of the sequence context, which is a potential shortcoming of antibody-based approaches. As a proof of concept, this method was successfully applied to the enrichment of tyrosine-nitrated angiotensin II in a tryptic digest of bovine serum albumin (BSA). The approach presented here is well adapted to peptide analysis, for instance in shotgun proteomics.
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